Structure of the Food-Poisoning Clostridium perfringens Enterotoxin Reveals Similarity to the Aerolysin-Like Pore-Forming Toxins
نویسندگان
چکیده
منابع مشابه
The Aerolysin-Like Toxin Family of Cytolytic, Pore-Forming Toxins
Pore-forming toxins (PFTs) represent the largest known group of bacterial protein toxins to date. Membrane insertion and subsequent pore-formation occurs after initial binding to cell-surface receptor and oligomerization. Aerolysin, a toxin produced by the Gram-negative bacterium Aeromonas hydrophila and related species, belongs to the PFT group and shares a common mechanism of action involving...
متن کاملOutbreak of Clostridium perfringens food poisoning.
An outbreak of diarrhoea occurred at a 647-bedded long-stay hospital from 11 to 14 June 1989. Fifty-eight elderly residents developed symptoms and there were two deaths. The organism was identified as Clostridium perfringens type A, serotype TW23. The source of the outbreak was found to be inadequately reheated minced beef served at lunchtime on 11 June. The reason why only 4 of the possible 16...
متن کاملA rivet model for channel formation by aerolysin-like pore-forming toxins.
The bacterial toxin aerolysin kills cells by forming heptameric channels, of unknown structure, in the plasma membrane. Using disulfide trapping and cysteine scanning mutagenesis coupled to thiol-specific labeling on lipid bilayers, we identify a loop that lines the channel. This loop has an alternating pattern of charged and uncharged residues, suggesting that the transmembrane region has a be...
متن کاملStructure of the claudin-binding domain of Clostridium perfringens enterotoxin.
Clostridium perfringens enterotoxin is a common cause of food-borne and antibiotic-associated diarrhea. The toxin's receptors on intestinal epithelial cells include claudin-3 and -4, members of a large family of tight junction proteins. Toxin-induced cytolytic pore formation requires residues in the NH(2)-terminal half, whereas residues near the COOH terminus are required for binding to claudin...
متن کاملIdentification and Characterization of a New Enterotoxin Produced by Clostridium perfringens Isolated from Food Poisoning Outbreaks
There is a strain of Clostridium perfringens, W5052, which does not produce a known enterotoxin. We herein report that the strain W5052 expressed a homologue of the iota-like toxin components sa and sb of C. spiroforme, named Clostridium perfringens iota-like enterotoxin, CPILE-a and CPILE-b, respectively, based on the results of a genome sequencing analysis and a systematic protein screening. ...
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ژورنال
عنوان ژورنال: Journal of Molecular Biology
سال: 2011
ISSN: 0022-2836
DOI: 10.1016/j.jmb.2011.07.066